IGVFFI6514JTIC

released

validated

File Set

IGVFDS8719WWWS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinD-PCR3_S124

md5sum

2ab130f984cc04c552972f29cbd39edc

Content MD5sum

d320befacfbc8057a4fb25e1f9ee7cf3

File Size

15 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinD-PCR3_S124_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTATGCC:ATTG

Average Read Length

Read Count

622010

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3068ZYXS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library