Home

IGVFFI6480QWYG

released
validated
File Set
IGVFDS4701EMZA(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep1-BinF-PCRrep1_R2
md5sum
bf06287e5cc0a37996fb9a433807e43c
Content MD5sum
9a7050b27d6a7b0e19f596eb26ad42ce
File Size
16 MB
Submitted File Name
220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep1-BinF-PCRrep1_S27_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
2
Illumina Read Type
R2
Index
CATAGCGA:ACTG
Average Read Length
150
Read Count
350826

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford