IGVFFI6480QLXO

released

validated

File Set

IGVFDS7007ZSGW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR2-Rep1_S43

md5sum

3ec3f1d2afe0f173c48b331a56e0c238

Content MD5sum

f8ce446bcd09ff26a3f0516bd45f137f

File Size

8.5 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF2-BinA-PCR2-Rep1_S43_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCATCTA:TTGA

Average Read Length

Read Count

390417

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2476YZDG

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library