IGVFFI6422IECE

preview
validated
1{
2    "@context": "/terms/",
3    "@id": "/sequence-files/IGVFFI6422IECE/",
4    "@type": [
5        "SequenceFile",
6        "File",
7        "Item"
8    ],
9    "accession": "IGVFFI6422IECE",
10    "aliases": [
11        "gary-hon:LW422B_6_I2"
12    ],
13    "assay_titles": [
14        "Perturb-seq"
15    ],
16    "audit": {},
17    "award": {
18        "@id": "/awards/HG011996/",
19        "component": "functional characterization"
20    },
21    "content_md5sum": "6b548302c48d74b3219a073909467f26",
22    "content_type": "reads",
23    "controlled_access": false,
24    "creation_timestamp": "2024-11-13T21:41:07.812831+00:00",
25    "externally_hosted": false,
26    "file_format": "fastq",
27    "file_set": {
28        "@id": "/auxiliary-sets/IGVFDS0170TMBA/",
29        "@type": [
30            "AuxiliarySet",
31            "FileSet",
32            "Item"
33        ],
34        "accession": "IGVFDS0170TMBA",
35        "file_set_type": "gRNA sequencing",
36        "samples": [
37            {
38                "@id": "/in-vitro-systems/IGVFSM8986CPUS/",
39                "@type": [
40                    "InVitroSystem",
41                    "Biosample",
42                    "Sample",
43                    "Item"
44                ],
45                "accession": "IGVFSM8986CPUS",
46                "classifications": [
47                    "differentiated cell specimen"
48                ],
49                "modifications": [
50                    {
51                        "@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
52                        "modality": "interference",
53                        "status": "preview",
54                        "summary": "CRISPRi Sp-dCas9"
55                    }
56                ],
57                "sample_terms": [
58                    {
59                        "@id": "/sample-terms/EFO_0003045/",
60                        "status": "released",
61                        "term_name": "H9"
62                    }
63                ],
64                "status": "preview",
65                "summary": "Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW422) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
66                "targeted_sample_term": {
67                    "@id": "/sample-terms/CL_0000746/",
68                    "status": "released",
69                    "term_name": "cardiac muscle cell"
70                },
71                "taxa": "Homo sapiens"
72            }
73        ],
74        "status": "preview",
75        "summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
76    },
77    "file_size": 1147661008,
78    "flowcell_id": "227C7WLT4",
79    "gene_list_for": [],
80    "href": "/sequence-files/IGVFFI6422IECE/@@download/IGVFFI6422IECE.fastq.gz",
81    "illumina_read_type": "I2",
82    "input_file_for": [
83        "/matrix-files/IGVFFI8522GXEK/",
84        "/matrix-files/IGVFFI0015OJLL/"
85    ],
86    "integrated_in": [],
87    "lab": {
88        "@id": "/labs/gary-hon/",
89        "title": "Gary Hon, UT"
90    },
91    "lane": 6,
92    "loci_list_for": [],
93    "maximum_read_length": 10,
94    "md5sum": "a0bc5577777234cd044d64423d25d78d",
95    "mean_read_length": 10,
96    "minimum_read_length": 10,
97    "quality_metrics": [],
98    "read_count": 167643252,
99    "s3_uri": "s3://igvf-files/2024/11/13/6c21e297-3ece-4375-a678-94e81d755289/IGVFFI6422IECE.fastq.gz",
100    "schema_version": "15",
101    "seqspecs": [],
102    "sequencing_kit": "NovaSeq X Series 25B Reagent Kit",
103    "sequencing_platform": {
104        "@id": "/platform-terms/EFO_0022841/",
105        "status": "released",
106        "term_name": "Illumina NovaSeq X Plus"
107    },
108    "sequencing_run": 1,
109    "status": "preview",
110    "submitted_by": {
111        "@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
112        "title": "Mpathi Nzima"
113    },
114    "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW422B_S2_L006_I2_001.fastq.gz",
115    "summary": "I2 reads from sequencing run 1",
116    "upload_status": "validated",
117    "uuid": "6c21e297-3ece-4375-a678-94e81d755289"
118}