IGVFFI6405ADNH

released

validated

File Set

IGVFDS3536DHAB(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispri-ccr7-high-bin-rep1-R1

md5sum

353d347460d1d564a3a6ba5391ad3cd6

Content MD5sum

b3c8345f71e10e070fb09c47f082e400

File Size

19 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/CCR7pos-pSM27-TF-H1_S2_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

000000000-K4RT4

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

805013

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2207SJEN

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)