IGVFFI6322UNED

released

validated

File Set

IGVFDS1409CHZY(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR1_S14

md5sum

006c22fd21f44d158db9cfd91bdca5ab

Content MD5sum

1b03fe4fab00159d2e4bae1f46473da8

File Size

13 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinB-PCR1_S14_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CACATCCT:CTCT

Average Read Length

Read Count

538230

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3263KXLP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library