IGVFFI6303UTLS

released
validated
File Set
IGVFDS7971RAUG(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF2-BinD-PCR1-Rep1_S40
md5sum
58f787629b0f47d8e1700a8af74ec300
Content MD5sum
39e7700b24ddd9dc5a0c609a317d3b94
File Size
11 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF2-BinD-PCR1-Rep1_S40_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
CCAGAGCT:TTGA
Average Read Length
21
Read Count
484040

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI6303UTLS
Jesse Engreitz, Stanford
912 B
validated

Attribution