IGVFFI6284GDRW

released

validated

File Set

IGVFDS0887BEDU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin6hrs-FF2-BinC-PCR1-Rep1_S147

md5sum

dd90413b204cc33b652111e8e397e64e

Content MD5sum

06289be44b38277ce50c70f9627ecad5

File Size

16 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin6hrs-FF2-BinC-PCR1-Rep1_S147_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CTGTAATC:CCAG

Average Read Length

Read Count

715106

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5674HHJF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library