IGVFFI6243FYBR

released

validated

File Set

IGVFDS0973WJFG(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF2-BinA-PCR1-Rep1_S13

md5sum

880e2b94d9ecb7e739396007f61f444c

Content MD5sum

9ca3d637851e1f3fcf3f2c7b6d5b8d6f

File Size

6.2 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF2-BinA-PCR1-Rep1_S13_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AACTTGAC:TTGA

Average Read Length

Read Count

282029

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1725FTKZ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library