IGVFFI6234KUHQ

released

validated

File Set

IGVFDS7971RAUG(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF2-BinD-PCR4-Rep1_S100

md5sum

ca0410132a6123debef803cd2be15d9f

Content MD5sum

b44bd951e5d3aef160472ea53ee8f24d

File Size

1.6 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF2-BinD-PCR4-Rep1_S100_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTGTCTG:GCCA

Average Read Length

Read Count

70027

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6811XGTM

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library