IGVFFI6175FLDC

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI6175FLDC/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI6175FLDC",
    "aliases": [
        "jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep1-BinD-PCRrep2_R1"
    ],
    "assay_titles": [
        "Variant-EFFECTS"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "88ecf76a66a67ae11cf9e1d315a8eb0c",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2023-12-05T18:38:40.094768+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/measurement-sets/IGVFDS6624WHBQ/",
        "accession": "IGVFDS6624WHBQ",
        "file_set_type": "experimental data",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM8604RBAL/",
                "accession": "IGVFSM8604RBAL",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                        "modality": "prime editing",
                        "status": "released",
                        "summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0001253/",
                        "status": "released",
                        "term_name": "THP-1"
                    }
                ],
                "status": "released",
                "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "released",
        "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
    },
    "file_size": 8359707,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI6175FLDC/@@download/IGVFFI6175FLDC.fastq.gz",
    "illumina_read_type": "R1",
    "index": "GCACATCT:TGAC",
    "input_file_for": [
        "/tabular-files/IGVFFI2325NRPY/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 150,
    "md5sum": "bc92b39c0b80e7c9759d5ab23ebbcba0",
    "mean_read_length": 150,
    "minimum_read_length": 150,
    "read_count": 369141,
    "release_timestamp": "2023-12-21T00:03:22.857613+00:00",
    "s3_uri": "s3://igvf-files/2023/12/05/7decbac7-84ff-45c1-bb8b-f1532bb11492/IGVFFI6175FLDC.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0008566/",
        "status": "released",
        "term_name": "Illumina NextSeq 550"
    },
    "sequencing_run": 2,
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep1-BinD-PCRrep2_S121_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 2",
    "upload_status": "validated",
    "uuid": "7decbac7-84ff-45c1-bb8b-f1532bb11492"
}