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INDEXED
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SequenceFile
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IGVFFI6072QDBA
IGVFFI6072QDBA
preview
validated
Json view
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4028IRAH
(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW283_2_I2
md5sum
497d0d314e50305dd1eca619a0564540
Content MD5sum
67421677d25a7303f5f22fa12cd19bfb
File Size
85 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW283_S21_L002_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Sequencing Details
Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7VLGHV
Lane
2
Illumina Read Type
I2
Average Read Length
10
Read Count
10810320
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM8464JMJS
InVitroSystem
GM25256 (WTC-11)
GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Files Derived From This File
2 items
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI8487QYZL
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
540 MB
validated
IGVFFI9503SXXQ
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
508 MB
validated
Attribution
Award
HG011996
Principal Investigator(s)
Bruce Posner
,
Gary Hon
,
William Kraus
,
Nikhil Munshi
,
Maria Chahrour
Contact P.I.
Gary Hon
Lab
Gary Hon, UT