IGVFFI6004KUNG

released

validated

File Set

IGVFDS7753HONE(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinE-PCR1_S17

md5sum

d85109f43d0b3a6eb2daa869a7a7e118

Content MD5sum

482b8c1ba4710777d789b0fe2d58957b

File Size

2.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinE-PCR1_S17_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCACACGA:CTCT

Average Read Length

Read Count

117435

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9074NPMT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library