IGVFFI5991TZXM

released

validated

File Set

IGVFDS5825GTXD(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes)

Summary

R1 reads from sequencing run 2

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispri-ccr7-low-bin-rep2-R1

md5sum

e7eb0bc763d6a62e8e40f022ae25998c

Content MD5sum

0a4ed5a4b5fa2c12623d3d812d93f493

File Size

13 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/CCR7pos-pSM27-TF-L3_S3_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

000000000-K4RT4

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

539802

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9398LTWW

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)