IGVFFI5991CNOJ

released

validated

File Set

IGVFDS8183BLZH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinE-PCR2-Rep1_S11

md5sum

ea18683498d9dc56b3248f260b262bbc

Content MD5sum

8d54e108812d2fedd1383e07c9fd40fd

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinE-PCR2-Rep1_S11_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTATAACA:TTGA

Average Read Length

Read Count

1023321

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7435FSSP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library