IGVFFI5884JDLP

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS5532ZGWU(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep2-FFrep1-BinD-PCRrep3_I1
md5sum
2e0b672e071c8923291e38193710cf13
Content MD5sum
2d93bdaec769b039f278a5ab22c3e672
File Size
5.0 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_pool-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinD-PCRrep3_S742_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
GCAGCCGT:GATATCGA
Average Read Length
8
Read Count
594832

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
pending

Attribution