IGVFFI5866ZGPN

released

validated

File Set

IGVFDS4902IYAA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinE-PCR2-Rep1_S251

md5sum

4963b7f1beb180de867afedf5a91b442

Content MD5sum

b460e1a3a21d8aff6cfb9c0d7cd2730d

File Size

14 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinE-PCR2-Rep1_S251_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GACCTAAC:TTGA

Average Read Length

Read Count

630070

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3721QHDS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library