IGVFFI5861BCEB

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI5861BCEB/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI5861BCEB",
    "assay_titles": [
        "CRISPR FlowFISH screen"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "c88c28e4982949ee5121b0a4850acb2b",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-04-16T22:02:49.763847+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS0020GFXZ/",
        "accession": "IGVFDS0020GFXZ",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM7134FIHW/",
                "accession": "IGVFSM7134FIHW",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
                        "modality": "interference",
                        "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/NTR_0000753/",
                        "term_name": "TeloHAEC"
                    }
                ],
                "summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3"
    },
    "file_size": 28247970,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI5861BCEB/@@download/IGVFFI5861BCEB.fastq.gz",
    "illumina_read_type": "R1",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 25,
    "md5sum": "a4535ccabb72ada8bb5feee22746e396",
    "mean_read_length": 25,
    "minimum_read_length": 25,
    "read_count": 1239340,
    "release_timestamp": "2024-06-22T00:27:02.270804+00:00",
    "s3_uri": "s3://igvf-files/2024/04/16/2ed8881c-3736-40f9-b269-8a2350553c52/IGVFFI5861BCEB.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "MiSeq Reagent Kit v2",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0004205/",
        "term_name": "Illumina MiSeq"
    },
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
        "title": "Chongyon Park"
    },
    "submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep2-FFrep2-BinD-PCRrep2_S220_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "2ed8881c-3736-40f9-b269-8a2350553c52"
}