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IGVFFI5825URTF

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validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3625ICZE(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinC-PCRrep1_R1
md5sum
b81b69eb8b6eac943ef04a8ec4954354
Content MD5sum
9bfdc6b5b4837813c8e2b797423167ec
File Size
15 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinC-PCRrep1_S921_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
R1
Index
AATGTTCT:TATGAGTA
Average Read Length
151
Read Count
662406

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford