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IGVFFI5824TPXK

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2536BETY(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW428B_6_I1
md5sum
4c31f5b9ba6cd1350e31b1f78a3b8daa
Content MD5sum
56a7b83b2dff0862c8ac3a6a20af2e09
File Size
599 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW428B_S14_L006_I1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I1
Average Read Length
10
Read Count
89247862

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW428) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated