IGVFFI5768PGXU

released
validated
File Set
IGVFDS6130MYTQ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinD-PCR1-Rep1_S244
md5sum
8468d10cf05a60033f6918b1a6304808
Content MD5sum
11a87f4045f259175bffc3ff61e36a5e
File Size
19 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinD-PCR1-Rep1_S244_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
TCGCTAGA:TTGA
Average Read Length
21
Read Count
863433

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI5768PGXU
Jesse Engreitz, Stanford
914 B
validated

Attribution