IGVFFI5756XBGF

released

validated

File Set

IGVFDS6449NYRM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR4-Rep1_S170

md5sum

13c22fa7b66bc0759fb735e1e8db9238

Content MD5sum

9a761830131ed464093925cddcc345a7

File Size

26 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinB-PCR4-Rep1_S170_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGGAGCC:ATTG

Average Read Length

Read Count

1193671

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0069TVXJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library