IGVFFI5750HKEP

released

validated

File Set

IGVFDS4580MCDT(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep2_S198

md5sum

01dbc911f591b6f0c63cb6b22da62a1d

Content MD5sum

84dd81e430f99882ed2e93bf45a2cd27

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinC-PCR2-Rep2_S198_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CGTTACCA:GCCA

Average Read Length

Read Count

1120374

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8399ZECZ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library