HG011972

Ian Whaling

Stanford Center for Connecting DNA Variants to Function and Phenotype

Jesse Engreitz

jesse-engreitz

Jesse Engreitz, Stanford

Anshul Kundaje

Marlene Rabinovitch

Lars Steinmetz

Michael Bassik

Thomas Quertermous

Will Greenleaf

audit_item_status

audit_missing_seqspec

audit_CRISPR_assay_missing_cell_sorting_auxiliary_set

audit_Variant_FlowFISH_missing_variant_sequencing_auxiliary_set

audit_targeted_genes

audit_inconsistent_sequencing_kit

Variant FlowFISH

Guide (sgRNA) library targeting sequence variants in a genomic locus

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours

Michael Montgomery

cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

IGVFFI5749XIDR

Sequencing Details

Attribution