IGVFFI5740TOCS

released

validated

File Set

IGVFDS2726RPEW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR2_S173

md5sum

cabc29e334f0c1e8f36e08be68a1e7e1

Content MD5sum

6bfc2a730b35c8c33659ae3d5fbc41df

File Size

7.5 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinE-PCR2_S173_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACAGTTGA:CATA

Average Read Length

Read Count

321902

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1617PVUY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library