Home

IGVFFI5735QBEZ

released
validated
File Set
IGVFDS9964KTTT(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep2_I1
md5sum
f043f93dd9039a1ee14f9368770fd411
Content MD5sum
12d09c92f4098a90e145d7cc91e3ee92
File Size
1.9 MB
Submitted File Name
230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep3_BinAll-PCRrep2_S390_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
TCCTTGGT:TTGT
Average Read Length
8
Read Count
243424

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford