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IGVFFI5730ESHZ

released
validated
File Set
IGVFDS4638GTKE(CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
richard-sherwood:B3.1_18loci_Rep3_bulk_read2
md5sum
610923d2ae055838f2da8907036db459
Content MD5sum
5b3acc87d5b3db8b1c774ee339980096
File Size
7.4 MB
Submitted File Name
/net/bgm/sherwood/Tian_demult_data/nextseq_051523_Tian/fixed/B3.1_18loci_Rep3_bulk_R2.fastq.gz
Checkfiles Version
v17

Sequencing Details

Sequencing Platform
Element Biosciences AVITI
Sequencing Run
1
Illumina Read Type
R2
Average Read Length
60
Read Count
221911

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
guide quantifications
Richard Sherwood, Brigham and Women's Hospital
336 KB
validated

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Richard Sherwood, Brigham and Women's Hospital