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IGVFFI5713MCUV

released
validated
File Set
IGVFDS9139TBCB(interference single-nucleus RNA sequencing assay (10x multiome with MULTI-seq))
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
ansuman-satpathy:igvf_exp10_rna_10x2_NGS2_r2
md5sum
99887d2f8662da3c64c7b09169f6e1b4
Content MD5sum
1f09d36f7f98da9a691e24cf01a7d7a7
File Size
22 GB
Submitted File Name
/oak/stanford/projects/igvf/Projects/230707_IGVF10_McGinnis/GEX/FASTQ/GEX_2_CMAS_IGVF10_R_S10_L005_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Flowcell ID
223JHYLT3
Lane
5
Sequencing Kit
NovaSeq X Series 10B Reagent Kit
Illumina Read Type
R2
Average Read Length
90
Read Count
519519616

Samples

1 item
Accession
Type
Sample Terms
Disease Terms
Summary
MultiplexedSample
multiplexed sample: Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
hdf5
raw feature barcode matrix
cell by gene in raw feature barcode matrix
Ansuman Satpathy, Stanford
275 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI5713MCUV, IGVFFI4378SNUH, IGVFFI1923SQPQ, IGVFFI9877ARCO
Ansuman Satpathy, Stanford
1.4 KB
validated

Attribution

Award
HG012076
Principal Investigator(s)
Contact P.I.
Ansuman Satpathy
Lab
Ansuman Satpathy, Stanford