IGVFFI5649PKQE

released

validated

File Set

IGVFDS6286FOBM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR2-Rep1_S8

md5sum

0ddcdcd66cb4bd70d131ae836c612d87

Content MD5sum

18ab9870e2a2e9a24963c47adbe04571

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinB-PCR2-Rep1_S8_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTGAGCCT:TTGA

Average Read Length

Read Count

1009939

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0430YSKQ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library