IGVFFI5619AUER

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI5619AUER/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI5619AUER",
    "aliases": [
        "jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinE-PCR3-Rep1_S287"
    ],
    "assay_titles": [
        "CRISPR FlowFISH screen"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "3b6ae340f5d02d28e89672dac24150dc",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-06-01T01:42:46.956510+00:00",
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS4902IYAA/",
        "accession": "IGVFDS4902IYAA",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM3721QHDS/",
                "accession": "IGVFSM3721QHDS",
                "classifications": [
                    "cell line"
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002824/",
                        "term_name": "HCT116"
                    }
                ],
                "summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
    },
    "file_size": 25749453,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI5619AUER/@@download/IGVFFI5619AUER.fastq.gz",
    "index": "ACAGTTGA:GCCA",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 21,
    "md5sum": "54b2ecccfd5bba8eb499d52772aeec50",
    "mean_read_length": 21,
    "minimum_read_length": 21,
    "read_count": 1172395,
    "release_timestamp": "2024-07-10T20:09:41.287438+00:00",
    "s3_uri": "s3://igvf-files/2024/06/01/0353b05e-c812-4aee-8d5d-3e2f9ef1a3cf/IGVFFI5619AUER.fastq.gz",
    "schema_version": "14",
    "seqspecs": [
        "/configuration-files/IGVFFI9366NHKY/"
    ],
    "sequencing_platform": "/platform-terms/EFO_0008565/",
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
        "title": "Benjamin Doughty"
    },
    "submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinE-PCR3-Rep1_S287_R1_001.fastq.gz",
    "summary": "reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "0353b05e-c812-4aee-8d5d-3e2f9ef1a3cf"
}