IGVFFI5610GEDM

released

validated

File Set

IGVFDS8871HVZU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinA-PCR2-Rep1_S115

md5sum

c905e40f36cb31cfd9d77d50010880a1

Content MD5sum

822119eade6851c08dcf346a00a65edd

File Size

24 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinA-PCR2-Rep1_S115_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CATAGCGA:CCAG

Average Read Length

Read Count

1123220

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5410WZKF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library