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IGVFFI5578GRYN

released
validated
File Set
IGVFDS4786QNDV(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-BinA-PCRrep2_R2
md5sum
814fa383e4851c2d2fd08d3d83516e76
Content MD5sum
84854caa9dfcf52b44a91dd3f4b1677b
File Size
77 MB
Submitted File Name
220228pPPIF-FFSample-6-D21-pPPIF-Amp-pPPIF-2-1-2-BinA_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
CAATAGTC:TGAC
Average Read Length
150
Read Count
1933158

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
2.4 KB
validated