IGVFFI5521JTXL

released

validated

File Set

IGVFDS4045JTCH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF4-BinC-PCR3_S183

md5sum

930629d4f3e3ad7fd0196dfd134702ac

Content MD5sum

5bb696a52ec0fc1199d35df07bd8fce8

File Size

13 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF4-BinC-PCR3_S183_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GATATCCA:ATTG

Average Read Length

Read Count

551109

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7739FHWE

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library