{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI5482SCRU/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI5482SCRU",
"aliases": [
"gary-hon:LW277B-3_2_I1"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011996/",
"component": "functional characterization"
},
"content_md5sum": "a7837bb3f2289a02417324e342e93329",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-03-01T17:06:22.530662+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS8280AMDA/",
"accession": "IGVFDS8280AMDA",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM7513BJDT/",
"accession": "IGVFSM7513BJDT",
"classifications": [
"differentiated cell specimen"
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"targeted_sample_term": {
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"status": "released",
"term_name": "cardiac muscle cell"
},
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
"file_size": 183560459,
"flowcell_id": "AAC7VLGHV",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI5482SCRU/@@download/IGVFFI5482SCRU.fastq.gz",
"illumina_read_type": "I1",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/gary-hon/",
"title": "Gary Hon, UT"
},
"lane": 2,
"loci_list_for": [],
"maximum_read_length": 10,
"md5sum": "d7e63d9882a81839c5a404b9f4d5d355",
"mean_read_length": 10,
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"s3_uri": "s3://igvf-files/2024/03/01/eff650ac-b7b2-4158-b9d6-2e9a1f492ec1/IGVFFI5482SCRU.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0010963/",
"status": "released",
"term_name": "Illumina NextSeq 2000"
},
"sequencing_run": 1,
"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW277B-3_S23_L002_I1_001.fastq.gz",
"summary": "I1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "eff650ac-b7b2-4158-b9d6-2e9a1f492ec1"
}