IGVFFI5430ZDLE

released
validated
File Set
IGVFDS6085VVNC(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep2-BinA-PCRrep1_R2
md5sum
40195f2e63294e0f491a94ef064cb453
Content MD5sum
09569711ff884024f56ffc7669eb3e7f
File Size
14 MB
Submitted File Name
220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S13-BioRep1-FFRep2-BinA-PCRrep1_S120_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R2
Index
GCCTAGCC:GTCA
Average Read Length
150
Read Count
408416

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution