IGVFFI5379RZFO

released

validated

File Set

IGVFDS1293ORGQ(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-FACS_Input-PCRrep2_I2

md5sum

5cac3d27c8f662e301bf62ed7a073ce0

Content MD5sum

c5db7a81a95152d6a33b1c6064e17e4e

File Size

14 MB

Submitted File Name

220228pPPIF-FFSample-6-D21-pPPIF-Amp-pPPIF-2-1-2-BinAll_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 550

Sequencing Run

1

Illumina Read Type

I2

Index

GTCGCCTT:TGAC

Average Read Length

4

Read Count

1877214

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford