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IGVFFI5374UBHB

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validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS9423AXCY(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinA-PCRrep2_R2
md5sum
51f3ce9728ff02c0f505d8a96b70a2f6
Content MD5sum
cdf55a39b517d5dddfa13b588f5a0789
File Size
12 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinA-PCRrep2_S931_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
R2
Index
CAGCAAGG:TATGAGTA
Average Read Length
151
Read Count
583333

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford