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IGVFFI5354EYBB

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1672IHVM(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW430B_6_I2
md5sum
388fdcd5be4dbbe5e02a447543bddd66
Content MD5sum
bc2fd72c95404083d16a2616e9a6cb9c
File Size
533 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW430B_S16_L006_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I2
Average Read Length
10
Read Count
71608939

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW430) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated