{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI5349UFOZ/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI5349UFOZ",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-FACS_Input-PCRrep2_R1"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "da8f88d6a3d1c0fb0cde3b7a8fc9cbb4",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:35:32.678237+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS2978YQVA/",
"accession": "IGVFDS2978YQVA",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5940EPFP/",
"accession": "IGVFSM5940EPFP",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"status": "released",
"term_name": "THP-1"
}
],
"status": "released",
"summary": "THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 4526436,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI5349UFOZ/@@download/IGVFFI5349UFOZ.fastq.gz",
"illumina_read_type": "R1",
"index": "CATGCTTA:TTGT",
"input_file_for": [
"/tabular-files/IGVFFI1480MKHV/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 150,
"md5sum": "24d9c3c88f8a2ac9fb372b0f8a404931",
"mean_read_length": 150,
"minimum_read_length": 150,
"read_count": 153990,
"release_timestamp": "2023-12-21T00:08:45.433225+00:00",
"s3_uri": "s3://igvf-files/2023/12/05/f18b0adc-828d-4299-9f76-24866d560178/IGVFFI5349UFOZ.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-BinAll-PCRrep2_S394_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "f18b0adc-828d-4299-9f76-24866d560178"
}