IGVFFI5330ZBZF

released

validated

File Set

IGVFDS4010VNGF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR2-Rep1_S128

md5sum

abc01fd85397ded3b0e0c93e66d0e529

Content MD5sum

ae0f2876afe570766c8f19586c7a30a1

File Size

13 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinB-PCR2-Rep1_S128_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCTAGG:CCAG

Average Read Length

Read Count

606893

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3569TFCR

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library