IGVFFI5292DWTM

released

validated

File Set

IGVFDS7400NQET(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep1_I2

md5sum

ebeffcbfe866432dcc6ae8730a8679f4

Content MD5sum

3e336cf585f8832ab325cc0ada30d3fc

File Size

14 MB

Submitted File Name

220228pPPIF-FFSample-4-D21-pPPIF-Amp-pPPIF-1-3-1-BinAll_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 550

Sequencing Run

1

Illumina Read Type

I2

Index

CTAACTCG:TGAC

Average Read Length

4

Read Count

1895517

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford