IGVFFI5281RKUY

released

validated

File Set

IGVFDS5688OXJC(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinC-PCR1_S15

md5sum

3f8c5522db417bd83f422dc66ee00d9d

Content MD5sum

cbad855813b4ce3de6f00ddf5ccd825d

File Size

6.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinC-PCR1_S15_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGGAATG:CTCT

Average Read Length

Read Count

286781

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7071BULA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library