{
"@context": "/terms/",
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],
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"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR1_Rep2_S190"
],
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "3269e461d353a7f97e7137e9a3001b3d",
"content_type": "reads",
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"creation_timestamp": "2024-06-01T02:06:34.291404+00:00",
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"file_format": "fastq",
"file_set": {
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"accession": "IGVFDS3773NYHJ",
"file_set_type": "gRNA sequencing",
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{
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"accession": "IGVFSM9689GGGO",
"classifications": [
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],
"modifications": [
{
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"modality": "degradation",
"summary": "AID system targeting RAD21"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
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{
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"term_name": "HCT116"
}
],
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
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{
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"purpose": "activation",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
}
],
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
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"href": "/sequence-files/IGVFFI5272TWLQ/@@download/IGVFFI5272TWLQ.fastq.gz",
"index": "GAAGAAGT:GCCA",
"input_file_for": [
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"integrated_in": [],
"lab": {
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"title": "Jesse Engreitz, Stanford"
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"maximum_read_length": 21,
"md5sum": "79ab3a91596d96434bd0de55bd282419",
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"release_timestamp": "2024-07-10T20:21:32.626954+00:00",
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"schema_version": "14",
"seqspecs": [
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],
"sequencing_platform": {
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"term_name": "Illumina HiSeq 2500"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
"title": "Benjamin Doughty"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinA-PCR1-Rep2_S190_R1_001.fastq.gz",
"summary": "reads from sequencing run 1",
"upload_status": "validated",
"uuid": "56666d9c-c3a3-437a-8954-77a5f6125169"
}