IGVFFI5243ZITD

released

validated

File Set

IGVFDS2850LCWI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR3_S198

md5sum

42b86effc4aae34e5eace7eb064e67a5

Content MD5sum

94630dbefac3cb080e68eed65e67dadf

File Size

3.3 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinF-PCR3_S198_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGTTGCTT:GTAC

Average Read Length

Read Count

139128

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0904FNLS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library