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IGVFFI5242ONUU

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4965SLDK(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW263_2_R2
md5sum
c57735813fd1ebae25f24be69f27388c
Content MD5sum
38a746df227e18b70799c2c69a246592
File Size
3.5 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-07-13-McDermott-NextSeq.10X.run3.transcriptome/2023-07-13/outs/fastq_path/AAC5GH5HV/LW263_S3_L002_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC5GH5HV
Lane
2
Illumina Read Type
R2
Average Read Length
92
Read Count
66700987

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
5.2 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
443 MB
validated