IGVFFI5230WISG

released
validated
File Set
IGVFDS6088VPGO(CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites genome-wide)
Summary
R2 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-rna-seq-cas-ko-nt-rep2-fastq-R2
md5sum
5974a1f3b415491414af203f49dc4d25
Content MD5sum
76dfdb95c061b041aae41209783d4c63
File Size
2.7 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM73-NT-2_R2_001.fastq.gz
Submitter Comment
Libraries prepared and sequenced by Genewiz. Unknown sequencing platform (best guess annotated)

Sequencing Details

Sequencing Platform
Sequencing Run
2
Lane
1
Illumina Read Type
R2
Average Read Length
150
Read Count
33576145

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.5 GB
validated

Attribution