IGVFFI5218FTCG

released

validated

File Set

IGVFDS4621HUKP(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jay-shendure:CRISPRa_singlecell_K562_VPR_sequence_CS1_10Xlane1_seqlane1_R2

md5sum

93c5e37de7664d2cb6c6632f4b4857c6

Content MD5sum

04d6a6bd0bc33fe5fe1b6a8872eaf1a9

File Size

1.4 GB

Submitted File Name

/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VPR_CS1_1/VPR_CS1_1_S7_L001_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 2000

Sequencing Run

Flowcell ID

AAAHG2HHV

Lane

Sequencing Kit

NextSeq 1000/2000 P3 Reagent Kit

Illumina Read Type

Average Read Length

Read Count

37512360

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1594ZOQH

InVitroSystem

K562

K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library