IGVFFI5212KCGT

released

validated

File Set

IGVFDS2160ZTSJ(CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes and an expression vector library of BATF3)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-high-bin-rep1-R1

md5sum

6394b82eae4677767aad43200235d328

Content MD5sum

2f9152f5790c64c7439d1420dd71f030

File Size

640 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM74_H2_S11_L001_R1_001.fastq.gz

Checkfiles Version

Legacy version of checkfiles

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

AACJJ7LM5

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

36430644

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8084QKPW

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)