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IGVFFI5212KCGT

released
validated
File Set
IGVFDS3370BGRA(gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-high-bin-rep1-R1
md5sum
6394b82eae4677767aad43200235d328
Content MD5sum
2f9152f5790c64c7439d1420dd71f030
File Size
640 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM74_H2_S11_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina MiSeq
Sequencing Run
1
Flowcell ID
AACJJ7LM5
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
21
Read Count
36430644

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
437 MB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke