IGVFFI5205NSJU

released

validated

File Set

IGVFDS4433BYLP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinD-PCR3-Rep1_S166

md5sum

d335f241f62f2ae08778ef4d9b1a48fa

Content MD5sum

45de8afaee29700a772464d27663d04f

File Size

34 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinD-PCR3-Rep1_S166_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AAGGATGT:ATTG

Average Read Length

Read Count

1585850

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7317UFYW

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library