IGVFFI5203ASJX

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS1248KTOA(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCCA-THP1-BioRep1-FFrep2-FACS_Input-PCRrep2_I2

md5sum

b1d5d8dfc86c1a0e8594c60a1ada9fb6

Content MD5sum

0bbd1cb33ef324cd612ad0e743b87ab7

File Size

12 MB

Submitted File Name

240908-S029-5splice-THP1-5splicesite_C10-Amp_PPIF_5splicesite-BioRep1-FFrep2-BinAll-PCRrep2_S980_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

1

Illumina Read Type

I2

Index

AGGTTATC:AGGTGCGT

Average Read Length

8

Read Count

1782273

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford